Doubled haploid technology in maize breeding: theory and practice
Prasanna, B.M.; Vijay Chaikam and George Mahuku
Abstract: This manual is primarily intended for maize breeders in the national agricultural research systems (NARS) and small and medium enterprise (SME) seed companies in the developing countries who would like to better underst and utilizes the doubled haploid (DH) technology in breeding programs. It is a compilation and consolidation of knowledge accumulated through scientific contributions of several maize geneticists and breeders worldwide as well as protocols successfully developed (in collaboration with University of Hohenheim, Germany) and being used by the CIMMYT Global Maize Program in DH line development, especially in Mexico. An overview of the utility and applications of DH technology in maize breeding is presented first in the Manual, followed by Chapters on in vivo maternal haploid induction using haploid inducers, haploid kernel detection using anthocyanin markers, chromosome doubling of haploids, deriving DH seed from colchicine‐treated plants, DH in commercial maize breeding, integrating molecular markers in DH‐based breeding pipeline, and finally, access to tropicalized haploid inducers and DH service from CIMMYT.