Posts Tagged ‘common wheat’

Characterization of Overexpressed Bx7 Gene (Bx7OE) in Chinese and CIMMYT Wheats by STS Markers

Posted by on , in Journal Articles

Published in Acta Agronomica Sinica 35(3): 403-411

Characterization of Overexpressed Bx7 Gene (Bx7OE) in Chinese and CIMMYT Wheats by STS Markers

Ren Yan, Liang Dan2, Zhang Ping-Ping, He Zhong-Hu, Chen Jing, Fu Ti-Hua, and Xia Xian-Chun

Over-expression of the high molecular weight glutenin subunit (HMW-GS) Bx7 is highly associated with dough strength of wheat (Triticum aestivum L.) flour. A total of 163 Chinese and CIMMYT wheat cultivars and advanced lines were tested by two STS markers and RP-HPLC to understand the presence of HMW-GS gene Bx7OE. The results indicated that the markers TaBAC1215C06-F517/R964 and TaBAC1215C06-F24671/R25515 could amplify a 447 bp and a 844 bp PCR fragments, respectively, in the lines with Bx7OE, whereas no PCR products were detected in the lines without Bx7OE. Of the 163 cultivars and lines, specific PCR fragments were amplified in 11 genotypes by the two markers, indicating the presence of Bx7OE in these lines, with a frequency of 6.7%. The results obtained by RP-HPLC were consistent with those revealed by STS markers. These two STS markers could be used to detect the presence of Bx7OE gene in wheat cultivars

Mapping of wheat stripe rust resistance gene YrZH84 with RGAP markers and its application

Posted by on , in Journal Articles

Published in Acta Agronomica Sinica 35(7): 1274-1281

Mapping of wheat stripe rust resistance gene and its application YrZH84 with RGAP markers and its application

Yin Gui-Hong, Wang Jian-Wu, Wen Wei-E2, He Zhong-Hu, Li Zai-Feng, Wang Hui and Xia Xian-Chun

Zhou 8425B, with an effective stripe rust resistance gene YrZH84, is a hall-mark parent line of wheat (Triticum aestivum L.) in the Yellow-Huai Rivers wheat growing region. An F2 population derived from the cross Zhou 8425B/Chinese Spring and their parents were used for polymorphism analysis employing the resistance gene-analog polymorphism (RGAP) markers and bulked segregant analysis (BSA) based on marker genotypes and phenotypes selection. An RGAP marker Xrga-1 was identified, which was tightly linked to the stripe rust resistance gene YrZH84, with a genetic distance of 0.8 cM. The fragment amplified by Xrga-1 was 343 bp. BLAST analysis indicated that the rga-1 showed 93% of identical nucleotide sequences to barley stem rust resistance gene Rpg1, and 92% to barley powdery mildew resistance gene Mla homology family. Fifty-eight cultivars from Yellow-Huai rivers wheat growing region were genotyped by the RGAP marker Xrga-1. From the information of genotypes, pedigrees and resistance responses, the cultivars Zhoumai 11, Zhoumai 20, Zhoumai 22, Aikang 58, 04 Zhong 36, Yuanyu 3, 05 Zhong 37, Wankang 18, and Yuzhan 10 were identified to carry the resistance gene YrZH84. These cultivars were the derivatives of Zhou 8425B. These results will greatly benefit the marker-assisted selection in wheat and cloning of the resistance gene YrZH84.