Characterization of genetic diversity of puroindoline genes in Mexican wheat landraces

Posted by Carelia Juarez on , in Journal Articles

Published in Euphytica, 2012

Marcela Ayala, Carlos Guzmán, Juan B. Alvarez and Roberto J. Peña

Grain hardness is a major factor determining milling performance of common wheat. It determines the amount of damaged starch generated during milling, and therefore the end use of a given variety. One hundred and two lines from 15 Mexican wheat landraces were analyzed for grain hardness and for its genetic control. Sixteen lines were hard and 86 were soft-textured. All hard lines could be explained by a mutation in either the PinaD1 or PinbD1 genes. In six hard lines there was no amplification of PinaD1, suggesting that this gene was deleted (PinaD1b allele). The remaining ten hard lines showed the presence of both PinaD1 and PinbD1. Sequencing the PinbD1 genes of the hard lines revealed the presence of two different alleles (PinbD1b and PinbD1e). The results substantiate the importance of very old Mexican landraces as potential sources of genetic diversity for key quality traits in the development of modern wheat cultivars with different grain textures.

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